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验证数据展示

  • WB analysis of mouse testis using 84189-4-RR (same clone as 84189-4-PBS)

    WB analysis of mouse testis using 84189-4-RR (same clone as 84189-4-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

  • WB analysis using 84189-4-RR (same clone as 84189-4-PBS)

    WB analysis using 84189-4-RR (same clone as 84189-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01062-1

    Cytometric bead array standard curve of MP01062-1

    Cytometric bead array standard curve of MP01062-1, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-1-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01062-2

    Cytometric bead array standard curve of MP01062-2

    Cytometric bead array standard curve of MP01062-2, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-2-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84189-4-RR

    Affinity and Kinetic Characterization of 84189-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

PIAS2 Recombinant antibody, PBS Only (Capture)

PIAS2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84189-4-PBS

产品说明书

CloneNo. 241474G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84189-4-PBS targets PIAS2 as part of a matched antibody pair:

MP01062-1: 84189-4-PBS capture and 84189-1-PBS detection (validated in Cytometric bead array)

MP01062-2: 84189-4-PBS capture and 84189-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PIAS2 fusion protein Ag9032 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein inhibitor of activated STAT, 2
别名 E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3
计算分子量 63 kDa, 69 kDa
观测分子量63-68 kDa
GenBank蛋白编号BC015190
基因名称 PIAS2
Gene ID (NCBI) 9063
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75928
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.