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验证数据展示

  • WB analysis using 82873-2-RR (same clone as 82873-2-PBS)

    WB analysis using 82873-2-RR (same clone as 82873-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82873-2-RR (PIAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIAS1 antibody included
  • IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIAS1 antibody included
  • IF Staining of HepG2 using 82873-2-RR (same clone as 82873-2-PBS)

    IF Staining of HepG2 using 82873-2-RR (same clone as 82873-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIAS1 antibody (82873-2-RR, Clone: 1C22 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82873-2-RR (same clone as 82873-2-PBS)

    FC experiment of HeLa using 82873-2-RR (same clone as 82873-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PIAS1 Recombinant antibody (82873-2-RR, Clone:1C22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82873-2-RR

    Affinity and Kinetic Characterization of 82873-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82873-2-RR against Human PIAS1 were performed. The affinity constant is 5.01 nM.

PIAS1 Recombinant monoclonal antibody, PBS Only

PIAS1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82873-2-PBS

产品说明书

CloneNo. 1C22

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PIAS1 Ready-To-Use IHC Kit. PIAS1 primary antibody included.

产品信息

82873-2-PBS targets PIAS1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18132

Product name: Recombinant human PIAS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 475-651 aa of BC121797

Sequence: PSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein inhibitor of activated STAT, 1
别名 EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1
计算分子量 651 aa, 72 kDa
观测分子量72 kDa
GenBank蛋白编号BC121797
基因名称 PIAS1
Gene ID (NCBI) 8554
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75925
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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