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验证数据展示

  • FC experiment of Jurkat using CL594-67071

    FC experiment of Jurkat using CL594-67071

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PI3 Kinase p110 Alpha (CL594-67071, Clone:1F6A7) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL594-67071

    IF Staining of HeLa using CL594-67071

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PI3 Kinase p110 Alpha antibody (CL594-67071, Clone: 1F6A7 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated PI3 Kinase p110 Alpha Monoclonal antibody

PI3 Kinase p110 Alpha Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67071

产品说明书

CloneNo. 1F6A7

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PIK3CA Ready-To-Use IHC Kit. PIK3CA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67071-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67071 targets PI3 Kinase p110 Alpha in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 PI3 Kinase p110 Alpha fusion protein Ag17392 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, catalytic, alpha polypeptide
别名 PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha
计算分子量 1068 aa, 124 kDa
观测分子量 110 kDa
GenBank蛋白编号BC113601
基因名称 PIK3CA
Gene ID (NCBI) 5290
RRIDAB_3084745
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42336
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

实验方案

Product Specific Protocols
IF protocol for CL594 PI3 Kinase p110 Alpha antibody CL594-67071Download protocol
Standard Protocols
Click here to view our Standard Protocols