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验证数据展示

  • WB analysis using 12267-1-AP (same clone as 12267-1-PBS)

    WB analysis using 12267-1-AP (same clone as 12267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

  • WB analysis using 12267-1-AP (same clone as 12267-1-PBS)

    WB analysis using 12267-1-AP (same clone as 12267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 12267-1-AP (same clone as 12267-1-PBS)

    IHC staining of human liver cancer using 12267-1-AP (same clone as 12267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PI16 antibody included
  • IHC staining of human pancreas cancer using 12267-1-AP (same clone as 12267-1-PBS)

    IHC staining of human pancreas cancer using 12267-1-AP (same clone as 12267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PI16 antibody included
  • IHC staining of human pancreas cancer using 12267-1-AP (same clone as 12267-1-PBS)

    IHC staining of human pancreas cancer using 12267-1-AP (same clone as 12267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PI16 antibody included
  • IHC staining of human liver cancer using 12267-1-AP (same clone as 12267-1-PBS)

    IHC staining of human liver cancer using 12267-1-AP (same clone as 12267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PI16 antibody included
  • Featured Product
  • KD/KO验证

PI16 Polyclonal antibody, PBS Only

PI16 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12267-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PI16 Ready-To-Use IHC Kit. PI16 primary antibody included.

产品信息

12267-1-PBS targets PI16 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2925

Product name: Recombinant human PI16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC022399

Sequence: MHGSCSFLMLLLPLLLLLVATTGPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLCEPIGSPEDAQDLPYLVTEAPSFRATEASDSRKMGTPSSLATGIPAFLVTEVSGSLATKALPAVETQAPTSLATKDPPSMATEAPPCVTTEVPSILAAHSLPSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidase inhibitor 16
别名 CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9
计算分子量 463 aa, 49 kDa
观测分子量49 kDa
GenBank蛋白编号BC022399
基因名称 PI16
Gene ID (NCBI) 221476
RRIDAB_10697651
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXB8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).

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