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验证数据展示

  • WB analysis using 12267-1-AP

    WB analysis using 12267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 12267-1-AP

    WB analysis using 12267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 12267-1-AP

    IHC staining of human liver cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PI16 antibody included
  • IHC staining of human pancreas cancer using 12267-1-AP

    IHC staining of human pancreas cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PI16 antibody included
  • IHC staining of human pancreas cancer using 12267-1-AP

    IHC staining of human pancreas cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PI16 antibody included
  • IHC staining of human liver cancer using 12267-1-AP

    IHC staining of human liver cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PI16 antibody included
  • Featured Product
  • KD/KO验证

PI16 Polyclonal antibody

PI16 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12267-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (2)

别名

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSKOV-3 cells, A2780 cells, COLO 320 cells, A549 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human pancreas cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PI16 Ready-To-Use IHC Kit. PI16 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12267-1-AP targets PI16 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag2925

Product name: Recombinant human PI16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC022399

Sequence: MHGSCSFLMLLLPLLLLLVATTGPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLCEPIGSPEDAQDLPYLVTEAPSFRATEASDSRKMGTPSSLATGIPAFLVTEVSGSLATKALPAVETQAPTSLATKDPPSMATEAPPCVTTEVPSILAAHSLPSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidase inhibitor 16
别名 CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9
计算分子量 463 aa, 49 kDa
观测分子量49 kDa
GenBank蛋白编号BC022399
基因名称 PI16
Gene ID (NCBI) 221476
RRIDAB_10697651
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).

实验方案

Product Specific Protocols
WB protocol for PI16 antibody 12267-1-APDownload protocol
IHC protocol for PI16 antibody 12267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IHC,CoIP

Cell Mol Biol Lett

Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO

Authors - Xiangqin Kuang
  • KD Validated
View Article
humanIF

Clin Cancer Res

Single-cell transcriptomic landscape deciphers intratumoral heterogeneity and subtypes of acral and mucosal melanoma

Authors - Yunyan Li
View Article