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验证数据展示

  • WB analysis using 16937-1-AP (same clone as 16937-1-PBS)

    WB analysis using 16937-1-AP (same clone as 16937-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16937-1-AP (PHYHIPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 16937-1-AP

    IHC staining of mouse brain using 16937-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)

    IF Staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 16937-1-AP (PHYHIPL antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)

    IHC staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.

PHYHIPL Polyclonal antibody, PBS Only

PHYHIPL Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 16937-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

KIAA1796, Phytanoyl-CoA hydroxylase-interacting protein-like, UNQ6309/PRO20934

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16937-1-PBS targets PHYHIPL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10435

Product name: Recombinant human PHYHIPL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-376 aa of BC011268

Sequence: MEVPRLDHALNSPTSPCEEVIKNLSLEAIQLCDRDGNKSQDSGIAEMEELPVPHNIKISNITCDSFKISWEMDSKSKDRITHYFIDLNKKENKNSNKFKHKDVPTKLVAKAVPLPMTVRGHWFLSPRTEYTVAVQTASKQVDGDYVVSEWSEIIEFCTADYSKVHLTQLLEKAEVIAGRMLKFSVFYRNQHKEYFDYVREHHGNAMQPSVKDNSGSHGSPISGKLEGIFFSCSTEFNTGKPPQDSPYGRYRFEIAAEKLFNPNTNLYFGDFYCMYTAYHYVILVIAPVGSPGDEFCKQRLPQLNSKDNKFLTCTEEDGVLVYHHAQDVILEVIYTDPVDLSLGTVAEITGHQLMSLSTANAKKDPSCKTCNISVGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phytanoyl-CoA 2-hydroxylase interacting protein-like
别名 KIAA1796, Phytanoyl-CoA hydroxylase-interacting protein-like, UNQ6309/PRO20934
计算分子量 376 aa, 43 kDa
观测分子量 39 kDa and 42-45 kDa
GenBank蛋白编号BC011268
基因名称 PHYHIPL
Gene ID (NCBI) 84457
RRIDAB_2163761
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96FC7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHYHIPL, a kind of mitochondrial protein, is associated with energy production, oxidative stress and organelle dynamics. It is reported that global ischemia is related to the change of PHYHIPL protein level. Compared with the control group, the level of APOE (may be involved in abnormal regulation of mitochondrial function) is significantly increased after ischemic stimulation, while the APOE genotype has a effect on PHYHIPL protein level. 16937-1-AP detects 39 kDa isoform and 42-45 kDa isoform in SDS-PAGE. (PMID: 21878944)

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