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验证数据展示

  • WB analysis using 16937-1-AP

    WB analysis using 16937-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16937-1-AP (PHYHIPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 16937-1-AP

    IF Staining of mouse brain using 16937-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 16937-1-AP (PHYHIPL antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

PHYHIPL Polyclonal antibody

PHYHIPL Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 16937-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

KIAA1796, PHYHIPL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16937-1-AP targets PHYHIPL in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PHYHIPL fusion protein Ag10435 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phytanoyl-CoA 2-hydroxylase interacting protein-like
别名 KIAA1796, PHYHIPL
计算分子量 376 aa, 43 kDa
观测分子量 39 kDa and 42-45 kDa
GenBank蛋白编号BC011268
基因名称 PHYHIPL
Gene ID (NCBI) 84457
RRIDAB_2163761
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96FC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHYHIPL, a kind of mitochondrial protein, is associated with energy production, oxidative stress and organelle dynamics. It is reported that global ischemia is related to the change of PHYHIPL protein level. Compared with the control group, the level of APOE (may be involved in abnormal regulation of mitochondrial function) is significantly increased after ischemic stimulation, while the APOE genotype has a effect on PHYHIPL protein level. 16937-1-AP detects 39 kDa isoform and 42-45 kDa isoform in SDS-PAGE. (PMID: 21878944)

实验方案

Product Specific Protocols
WB protocol for PHYHIPL antibody 16937-1-APDownload protocol
IHC protocol for PHYHIPL antibody 16937-1-APDownload protocol
IF protocol for PHYHIPL antibody 16937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols