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验证数据展示

  • WB analysis of A431 using 27940-1-AP (same clone as 27940-1-PBS)

    WB analysis of A431 using 27940-1-AP (same clone as 27940-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 27940-1-AP (PHLDB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 27940-1-AP (same clone as 27940-1-PBS)

    IHC staining of human placenta using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27940-1-AP (same clone as 27940-1-PBS)

    IHC staining of mouse heart using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

    IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

    IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

PHLDB2 Polyclonal antibody, PBS Only

PHLDB2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27940-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

Protein LL5-beta, Pleckstrin homology-like domain family B member 2, LL5beta, LL5B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27940-1-PBS targets PHLDB2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27331

Product name: Recombinant human PHLDB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC142678

Sequence: MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleckstrin homology-like domain, family B, member 2
别名 Protein LL5-beta, Pleckstrin homology-like domain family B member 2, LL5beta, LL5B
计算分子量 1253 aa, 142 kDa
观测分子量150 kDa
GenBank蛋白编号BC142678
基因名称 PHLDB2
Gene ID (NCBI) 90102
RRIDAB_2935465
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86SQ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The pleckstrin homology-like domain (PHLD) class of proteins are multifunctional proteins comprised of two families of proteins, PHLDA and PHLDB, each with 3 members: PHLDA1, PHLDA2, PHDLA3, PHLDB1, PHLDB2, and PHLDB3 (PMID: 32429563). All the family members possess a pleckstrin homology (PH) domain. PHLB2, also known as LL5-beta, contains two predicted coiled-coil domains, a serine-rich region predicted to contain multiple protein kinase C phosphorylation sites in addition to a PH domain (PMID: 15851520). PHLB2 is a filamin-binding protein that is an effector of the PI3K signaling pathway (PMID: 17174070). It is a PtdIns(3,4,5)P3 (PIP3) binding protein and can translocate to the plasma membrane in a receptor and PI3K-dependant manner (PMID: 12376540). PHLB2 has also been reported as a component of the postsynaptic apparatus and a regulator of postsynaptic differentiation (PMID: 15851520).

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