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验证数据展示

  • WB analysis of Jurkat using 24624-1-AP (same clone as 24624-1-PBS)

    WB analysis of Jurkat using 24624-1-AP (same clone as 24624-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24624-1-AP (PHF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24624-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 24624-1-AP (same clone as 24624-1-PBS)

    IP experiment of HeLa using 24624-1-AP (same clone as 24624-1-PBS)

    IP result of anti-PHF2 (IP:24624-1-AP, 4ug; Detection:24624-1-AP 1:500) with HeLa cells lysate 1800ug. This data was developed using the same antibody clone with 24624-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 24624-1-AP (same clone as 24624-1-PBS)

    IHC staining of human cervical cancer using 24624-1-AP (same clone as 24624-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24624-1-AP (PHF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24624-1-PBS in a different storage buffer formulation.

PHF2 Polyclonal antibody, PBS Only

PHF2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 24624-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24624-1-PBS targets PHF2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20257

Product name: Recombinant human PHF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 445-780 aa of BC152437

Sequence: ASKAVRPEVNTVASSDEVCDGDREKEEPPSPIEATPPQSLLEKVSKKKTPKTVKMPKPSKIPKPPKPPKPPRPPKTLKLKDGGKKKGKKSRESASPTIPNLDLLEAHTKEALTKMEPPKKGKATKSVLSVPNKDVVHMQNDVERLEIREQTKSKSEAKWKYKNSKPDSLLKMEEEQKLEKSPLAGNKDNKFSFSFSNKKLLGSKALRPPTSPGVFGALQNFKEDKPKPVRDEYEYVSDDGELKIDEFPIRRKKNAPKRDLSFLLDKKAVLPTPVTKPKLDSAAYKSDDSSDEGSLHIDTDTKPGRNARVKKESGSSAAGILDLLQASEEVGALEYN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 2
别名
计算分子量 1101 aa, 121 kDa
观测分子量 150 kDa
GenBank蛋白编号BC152437
基因名称 PHF2
Gene ID (NCBI) 5253
RRIDAB_2879644
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75151
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHD finger protein 2 (PHF2), also known as JHDM1E (Jumonji C domain-containing histone demethylase 1E) or GRC5, is a 1101 amino acid protein, which contains one JmjC domain and one PHD-type zinc finger. PHF2 belongs to the JHDM1 histone demethylase family and localizes in the nucleus. PHF2 demethylases both histones and non-histione proteins. PHF2 is widely expressed in various tissues and exists as two alternative splicing isoforms. The gene encoding PHF2 lies in the candidate region for hereditary sensory neuropathy type I (HSN1), a disorder characterized by sensory dysfunction

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