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PHF2 Polyclonal antibody

PHF2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24624-1-AP

Print datasheet

Synonyms

GRC5, JHDM1E, KIAA0662, PHD finger protein 2, PHF2

验证数据展示

  • WB analysis of Jurkat using 24624-1-AP

    WB analysis of Jurkat using 24624-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24624-1-AP (PHF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24624-1-AP

    IP experiment of HeLa using 24624-1-AP

    IP result of anti-PHF2 (IP:24624-1-AP, 4ug; Detection:24624-1-AP 1:500) with HeLa cells lysate 1800ug.

  • IHC staining of human cervical cancer using 24624-1-AP

    IHC staining of human cervical cancer using 24624-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24624-1-AP (PHF2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24624-1-AP is PHF2 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PHF2 fusion protein Ag20257 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PHD finger protein 2
Synonyms GRC5, JHDM1E, KIAA0662, PHD finger protein 2, PHF2
Calculated Molecular Weight 1101 aa, 121 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC152437
Gene Symbol PHF2
Gene ID (NCBI) 5253
RRIDAB_2879644
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHD finger protein 2 (PHF2), also known as JHDM1E (Jumonji C domain-containing histone demethylase 1E) or GRC5, is a 1101 amino acid protein, which contains one JmjC domain and one PHD-type zinc finger. PHF2 belongs to the JHDM1 histone demethylase family and localizes in the nucleus. PHF2 demethylases both histones and non-histione proteins. PHF2 is widely expressed in various tissues and exists as two alternative splicing isoforms. The gene encoding PHF2 lies in the candidate region for hereditary sensory neuropathy type I (HSN1), a disorder characterized by sensory dysfunction

实验方案

Product Specific Protocols
WB protocol for PHF2 antibody 24624-1-APDownload protocol
IHC protocol for PHF2 antibody 24624-1-APDownload protocol
IP protocol for PHF2 antibody 24624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Hum Genet

High-throughput identification of regulatory elements and functional assays to uncover susceptibility genes for nasopharyngeal carcinoma

Authors - Tong-Min Wang
View Article