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验证数据展示

  • WB analysis using 28472-1-AP (same clone as 28472-1-PBS)

    WB analysis using 28472-1-AP (same clone as 28472-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 28472-1-AP (same clone as 28472-1-PBS)

    WB analysis of HepG2 using 28472-1-AP (same clone as 28472-1-PBS)

    WB result of PHF17 antibody (28472-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF17 transfected HepG2 cells. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 28472-1-AP (same clone as 28472-1-PBS)

    IF Staining of HeLa using 28472-1-AP (same clone as 28472-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF17 antibody (28472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PHF17 Polyclonal antibody, PBS Only

PHF17 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28472-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Jade family PHD finger protein 1, JADE1, PHF 17, Protein Jade-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PHF17 Ready-To-Use IHC Kit. PHF17 primary antibody included.

产品信息

28472-1-PBS targets PHF17 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29675

Product name: Recombinant human PHF17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 360-450 aa of BC032376

Sequence: KFKSYCPKHSSHRKPEESLGKGAAQENGAPECSPRNPLEPFASLEQNREEAHRVSVRKQKLQQLEDEFYTFVNLLDVARALRLPEEVVDFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 17
别名 Jade family PHD finger protein 1, JADE1, PHF 17, Protein Jade-1
计算分子量 96 kDa
观测分子量65 kDa, 100-110 kDa
GenBank蛋白编号BC032376
基因名称 PHF17
Gene ID (NCBI) 79960
RRIDAB_2918168
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IE81
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHF17, also named as JADE1 and KIAA1807, belongs to the JADE family. PHF17 codes for the Jade-1 protein. This protein interacts with histone acetyltransferase HBO1 and promotes histone acetylation in chromatin to regulate DNA replication and gene transcription. PHF17 is mostly expressed in the small intestine, prostate, mammary gland and kidney. PHF17 is involved in mitosis, meiosis and tumor suppression (PMID: 28134766). PHF17 has 3 isoforms with the molecular mass of 58 kDa and 94-96 kDa. With modification, the MW of PHF17 may be migrated to about 65 kDa and 100-110 kDa.

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