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验证数据展示

  • WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

    WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGM1 antibody included
  • IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGM1 antibody included
  • IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

    IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

PGM1 Monoclonal antibody, PBS Only

PGM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66105-1-PBS

产品说明书

CloneNo. 2G5A8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2G5A8, CDG1T, EC:5.4.2.2, GSD14, Phosphoglucomutase-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.

产品信息

66105-1-PBS targets PGM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag7294

Product name: Recombinant human PGM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 213-562 aa of BC001756

Sequence: SGPNRLKICIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKHGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoglucomutase 1
别名 2G5A8, CDG1T, EC:5.4.2.2, GSD14, Phosphoglucomutase-1
计算分子量 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC001756
基因名称 PGM1
Gene ID (NCBI) 5236
RRIDAB_2881504
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP36871
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

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