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PGLYRP2 Polyclonal antibody

PGLYRP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 23029-1-AP

Print datasheet

Synonyms

HMFT0141, PGLYRP2, PGLYRPL, PGRP L, PGRPL, tagL, tagL alpha, tagl beta, TAGL like

验证数据展示

  • WB analysis of human plasma using 23029-1-AP

    WB analysis of human plasma using 23029-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 23029-1-AP

    IP experiment of human plasma using 23029-1-AP

    IP result of anti-PGLYRP2 (IP:23029-1-AP, 4ug; Detection:23029-1-AP 1:1000) with human plasma lysate 4000ug.

  • IHC staining of human liver cancer using 23029-1-AP

    IHC staining of human liver cancer using 23029-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 23029-1-AP

    IHC staining of human liver cancer using 23029-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23029-1-AP is PGLYRP2 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen PGLYRP2 fusion protein Ag19336 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidoglycan recognition protein 2
Synonyms HMFT0141, PGLYRP2, PGLYRPL, PGRP L, PGRPL, tagL, tagL alpha, tagl beta, TAGL like
Calculated Molecular Weight 576 aa, 62 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC136551
Gene Symbol PGLYRP2
Gene ID (NCBI) 114770
RRIDAB_2879199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96PD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidoglycan recognition protein 2 (PGLYRP2) is an N-acetylmuramoyl-L-alanine amidase that hydrolyzes peptidoglycan, a ubiquitous component of bacterial cell walls (PMID: 14506276). It is involved in innate immunity. PGLYRP2 is primarily expressed in liver and secreted into blood (PMID: 11461926). The apparent molecular mass measured by SDS-PAGE is lager than calculated mass of 62 kDa, which is likely due to N-glycosylation (PMID: 16054449).

实验方案

Product Specific Protocols
WB protocol for PGLYRP2 antibody 23029-1-APDownload protocol
IHC protocol for PGLYRP2 antibody 23029-1-APDownload protocol
IP protocol for PGLYRP2 antibody 23029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols