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验证数据展示

  • WB analysis of human urine using 85610-4-RR

    WB analysis of human urine using 85610-4-RR

    human urine sample were subjected to SDS PAGE followed by western blot with 85610-4-RR (PGLYRP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 85610-4-RR

    IHC staining of mouse spleen using 85610-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85610-4-RR (PGLYRP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 85610-4-RR

    IF Staining of mouse spleen using 85610-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using PGLYRP1 antibody (85610-4-RR, Clone: 243004F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 85610-4-RR

    IF Staining of mouse spleen using 85610-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using PGLYRP1 antibody (85610-4-RR, Clone: 243004F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PGLYRP1 Recombinant antibody

PGLYRP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85610-4-RR

产品说明书

CloneNo. 243004F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

PGLYRP, PGRP, PGRP S, PGRPS, PGRP-S

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman urine
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85610-4-RR targets PGLYRP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 PGLYRP1 fusion protein Eg3122 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 peptidoglycan recognition protein 1
别名 PGLYRP, PGRP, PGRP S, PGRPS, PGRP-S
计算分子量22 kDa
观测分子量25 kDa
GenBank蛋白编号NM_005091.2
基因名称 PGLYRP1
Gene ID (NCBI) 8993
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75594
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidoglycan recognition protein 1 (PGLYRP1, also known as PGRP-S) belongs to a family of evolutionary conserved innate immunity proteins, which in mammals also include PGLYRP2, PGLYRP3, and PGLYRP4 (PMID: 17275309; 20418257). PGLYRP1 is highly expressed in polymorphonuclear leukocytes and to a lower extent in many non-immune cells (PMID: 20418257). It recognizes bacterial peptidoglycan and functions in antibacterial immunity and inflammation (PMID: 20418257). PGLYRP1 has been identified as a ligand for TREM-1 (PMID: 25595774).

实验方案

Product Specific Protocols
WB protocol for PGLYRP1 antibody 85610-4-RRDownload protocol
IHC protocol for PGLYRP1 antibody 85610-4-RRDownload protocol
IF protocol for PGLYRP1 antibody 85610-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols