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验证数据展示

  • WB analysis using 18046-1-AP

    WB analysis using 18046-1-AP

    Rat brain tissue was subjected to SDS PAGE followed by western blot with 18046-1-AP (PGLYRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18046-1-AP (PGLYRP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18046-1-AP (PGLYRP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PGLYRP1 Polyclonal antibody

PGLYRP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18046-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SBBI68, PGRP-S, PGRPS, PGRP S, PGRP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18046-1-AP targets PGLYRP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PGLYRP1 fusion protein Ag12690 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidoglycan recognition protein 1
别名 SBBI68, PGRP-S, PGRPS, PGRP S, PGRP
计算分子量 196 aa, 22 kDa
观测分子量25 kDa
GenBank蛋白编号BC101845
基因名称 PGLYRP1
Gene ID (NCBI) 8993
RRIDAB_3085550
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75594
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidoglycan recognition protein 1 (PGLYRP1, also known as PGRP-S) belongs to a family of evolutionary conserved innate immunity proteins, which in mammals also include PGLYRP2, PGLYRP3, and PGLYRP4 (PMID: 17275309; 20418257). PGLYRP1 is highly expressed in polymorphonuclear leukocytes and to a lower extent in many non-immune cells (PMID: 20418257). It recognizes bacterial peptidoglycan and functions in antibacterial immunity and inflammation (PMID: 20418257). PGLYRP1 has been identified as a ligand for TREM-1 (PMID: 25595774).

实验方案

Product Specific Protocols
WB protocol for PGLYRP1 antibody 18046-1-APDownload protocol
IHC protocol for PGLYRP1 antibody 18046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols