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验证数据展示

  • WB analysis using 18046-1-AP

    WB analysis using 18046-1-AP

    Rat brain tissue was subjected to SDS PAGE followed by western blot with 18046-1-AP (PGLYRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18046-1-AP (PGLYRP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18046-1-AP (PGLYRP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PGLYRP1 Polyclonal antibody

PGLYRP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18046-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SBBI68, PGRP-S, PGRPS, PGRP S, PGRP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18046-1-AP targets PGLYRP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12690

Product name: Recombinant human PGLYRP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-196 aa of BC101845

Sequence: MSRRSMLLAWALPSLLRLGAAQETEDPACCSPIVPRNEWKALASECAQHLSLPLRYVVVSHTAGSSCNTPASCQQQARNVQHYHMKTLGWCDVGYNFLIGEDGLVYEGRGWNFTGAHSGHLWNPMSIGISFMGNYMDRVPTPQAIRAAQGLLACGVAQGALRSNYVLKGHRDVQRTLSPGNQLYHLIQNWPHYRSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidoglycan recognition protein 1
别名 SBBI68, PGRP-S, PGRPS, PGRP S, PGRP
计算分子量 196 aa, 22 kDa
观测分子量25 kDa
GenBank蛋白编号BC101845
基因名称 PGLYRP1
Gene ID (NCBI) 8993
RRIDAB_3085550
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75594
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidoglycan recognition protein 1 (PGLYRP1, also known as PGRP-S) belongs to a family of evolutionary conserved innate immunity proteins, which in mammals also include PGLYRP2, PGLYRP3, and PGLYRP4 (PMID: 17275309; 20418257). PGLYRP1 is highly expressed in polymorphonuclear leukocytes and to a lower extent in many non-immune cells (PMID: 20418257). It recognizes bacterial peptidoglycan and functions in antibacterial immunity and inflammation (PMID: 20418257). PGLYRP1 has been identified as a ligand for TREM-1 (PMID: 25595774).

实验方案

Product Specific Protocols
IHC protocol for PGLYRP1 antibody 18046-1-APDownload protocol
WB protocol for PGLYRP1 antibody 18046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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