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验证数据展示

  • WB analysis using 68035-1-Ig (same clone as 68035-1-PBS)

    WB analysis using 68035-1-Ig (same clone as 68035-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68035-1-Ig (PGK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGK1 antibody included
  • IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGK1 antibody included
  • IF Staining of A431 using 68035-1-Ig (same clone as 68035-1-PBS)

    IF Staining of A431 using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PGK1 antibody (68035-1-Ig, Clone: 1B2F3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

PGK1 Monoclonal antibody, PBS Only

PGK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68035-1-PBS

产品说明书

CloneNo. 1B2F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PRP 2, PGKA, OK/SW-cl.110, EC:2.7.2.3, Cell migration-inducing gene 10 protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PGK1 Ready-To-Use IHC Kit. PGK1 primary antibody included.

产品信息

68035-1-PBS targets PGK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12099

Product name: Recombinant human PGK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-417 aa of BC103752

Sequence: MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoglycerate kinase 1
别名 PRP 2, PGKA, OK/SW-cl.110, EC:2.7.2.3, Cell migration-inducing gene 10 protein
计算分子量 417 aa, 45 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC103752
基因名称 PGK1
Gene ID (NCBI) 5230
RRIDAB_2918777
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP00558
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).

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