PFTK1 Polyclonal antibody

PFTK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21612-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

CDK14, hPFTAIRE1, KIAA0834, PFTAIRE protein kinase 1, PFTAIRE1, PFTK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, SH-SY5Y cells
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CDK14/PFTK1 Ready-To-Use IHC Kit. CDK14/PFTK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21612-1-AP targets PFTK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 PFTK1 fusion protein Ag16259 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PFTAIRE protein kinase 1
别名 CDK14, hPFTAIRE1, KIAA0834, PFTAIRE protein kinase 1, PFTAIRE1, PFTK1
计算分子量 469 aa, 53 kDa
观测分子量 48-53 kDa
GenBank蛋白编号BC136477
基因名称 PFTK1
Gene ID (NCBI) 5218
RRIDAB_10858924
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94921
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFTK1 is a Cdc2-related protein kinase, also named as PFTAIRE1, CDK14 and belongs to the CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It acts as a cyclin-dependent kinases that regulates cell cycle progression and cell proliferation. The PFTK1 protein shows high homology with mouse Pftaire1, a Cdc2-related protein expressed primarily in the postnatal and adult nervous system(PMID:9202329). The protein contains 2 predicted nuclear localization signals that the N-terminal nuclear localization signals do not play a role in vivo(PMID:11313143). It has 3 isoforms produced by alternative splicing. This antibody is specific to PFTK1.

实验方案

Product Specific Protocols
WB protocol for PFTK1 antibody 21612-1-APDownload protocol
IHC protocol for PFTK1 antibody 21612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Commun Biol

Methylation-modulated PFTK1 regulates gefitinib resistance via Wnt/β-catenin signaling in EGFR mutant non-small-cell lung cancer cells

Authors - Xiaoting Jia
humanWB

Mol Ther Oncolytics

Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus

Authors - Dakota W. Goad
mouseWB, IHC, IF

Toxicol Lett

CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.

Authors - Daniel Pollack
humanWB

World Neurosurg

MiR-205 Inhibits Sporadic Vestibular Schwannoma Cell Proliferation by Targeting Cyclin-Dependent Kinase 14.

Authors - Xiaoling Yin
humanWB,IHC

Biomed Pharmacother

MiR-542-3p, a microRNA targeting CDK14, suppresses cell proliferation, invasiveness, and tumorigenesis of epithelial ovarian cancer.

Authors - Jingwei Li
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