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验证数据展示

  • WB analysis using 29902-1-AP (same clone as 29902-1-PBS)

    WB analysis using 29902-1-AP (same clone as 29902-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29902-1-AP (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 29902-1-AP (same clone as 29902-1-PBS)

    IHC staining of human kidney using 29902-1-AP (same clone as 29902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 29902-1-AP (same clone as 29902-1-PBS)

    IHC staining of human kidney using 29902-1-AP (same clone as 29902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 29902-1-AP (same clone as 29902-1-PBS)

    IF Staining of HUVEC using 29902-1-AP (same clone as 29902-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using PFKFB4 antibody (29902-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

PFKFB4 Polyclonal antibody, PBS Only

PFKFB4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29902-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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产品信息

29902-1-PBS targets PFKFB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30571

Product name: Recombinant human PFKFB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-232 aa of NM_001317134

Sequence: ATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVAD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
别名 6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4
计算分子量 54 kDa
观测分子量54 kDa
GenBank蛋白编号NM_001317134
基因名称 PFKFB4
Gene ID (NCBI) 5210
RRIDAB_2935486
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16877
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), is a bi-function enzyme. On one hand, PFKFB4 synthesizes fructose 2,6-bisphosphate (F2,6-BP) to stimulate glycolysis, playing important roles in metabolic processes. On the other hand, PFKFB4 acts as a phosphatase to hydrolyze F2,6-BP into fructose-6-phosphate (F6P) and inorganic phosphate (Pi), functioning as a protein kinase to phosphorylate protein substrates. The dual function of PFKFB4 makes it involved in multiple biological processes such as cell cycle regulation, cell proliferation and transcriptional regulation(PMID: 33593309). PFKFB4 has been reported to be involved in a variety of cancers including human bladder cancer, gastric cancer, colon cancer, breast cancer and other malignant tumors(PMID: 21396842, PMID: 33732372, PMID: 31244553).

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