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CoraLite®555-conjugated PEX14 Recombinant antibody

PEX14 Recombinant Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

7E6

Cat No : CL555-80234

Print datasheet

Synonyms

dJ734G22.2, NAPP2, Peroxin 14, PEX14, Pex14p, PTS1 receptor docking protein

验证数据展示

  • IF Staining of HeLa using CL555-80234

    IF Staining of HeLa using CL555-80234

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 PEX14 antibody (CL555-80234, Clone: 7E6 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-80234

    FC experiment of HeLa using CL555-80234

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human PEX14 (CL555-80234, Clone:7E6) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 80234-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-80234 targets PEX14 in IF, FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, rat
Immunogen PEX14 fusion protein Ag0932 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name peroxisomal biogenesis factor 14
Synonyms dJ734G22.2, NAPP2, Peroxin 14, PEX14, Pex14p, PTS1 receptor docking protein
Calculated Molecular Weight 41 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC006327
Gene Symbol PEX14
Gene ID (NCBI) 5195
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75381
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

实验方案

Product Specific Protocols
IF protocol for CL555 PEX14 antibody CL555-80234Download protocol
FC protocol for CL555 PEX14 antibody CL555-80234Download protocol
Standard Protocols
Click here to view our Standard Protocols