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验证数据展示

  • WB analysis using 16097-1-AP (same clone as 16097-1-PBS)

    WB analysis using 16097-1-AP (same clone as 16097-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16097-1-AP (PELI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16097-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16097-1-AP (same clone as 16097-1-PBS)

    IF Staining of HepG2 using 16097-1-AP (same clone as 16097-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PELI2 antibody (16097-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16097-1-PBS in a different storage buffer formulation.

PELI2 Polyclonal antibody, PBS Only

PELI2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16097-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

E3 ubiquitin-protein ligase pellino homolog 2, EC:2.3.2.27, Pellino 2, Pellino-2, Protein pellino homolog 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16097-1-PBS targets PELI2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8940

Product name: Recombinant human PELI2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-420 aa of BC009476

Sequence: RPQCPVGLNTLAFPSINRKEVVEEKQPWAYLSCGHVHGYHNWGHRSDTEANERECPMCRTVGPYVPLWLGCEAGFYVDAGPPTHAFTPCGHVCSEKSAKYWSQIPLPHGTHAFHAACPFCATQLVGEQNCIKLIFQGPID

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pellino homolog 2 (Drosophila)
别名 E3 ubiquitin-protein ligase pellino homolog 2, EC:2.3.2.27, Pellino 2, Pellino-2, Protein pellino homolog 2
计算分子量 420 aa, 46 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC009476
基因名称 PELI2
Gene ID (NCBI) 57161
RRIDAB_2878218
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAT8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pellino2 (PELI2) is a newly discovered E3 ubiquitin ligase, which regulates the protein degradation, protein-protein interaction, protein translocation and signaling transduction via the ubiquitination of target proteins, and plays important roles in inflammation and the immune system. PELI2 possesses a C-terminal RING-like domain and a phospho-threonine-binding forkhead-associated (FHA) domain that are responsible for ubiquitin ligase activity and substrate binding, respectively (PMID: 24699078).IHC

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