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验证数据展示

  • WB analysis using 16097-1-AP

    WB analysis using 16097-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16097-1-AP (PELI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 16097-1-AP

    IHC staining of mouse ovary using 16097-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16097-1-AP (PELI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16097-1-AP

    IF Staining of HepG2 using 16097-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PELI2 antibody (16097-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PELI2 Polyclonal antibody

PELI2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16097-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

RING-type E3 ubiquitin transferase pellino homolog 2, Protein pellino homolog 2, Pellino-2, Pellino 2, EC:2.3.2.27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16097-1-AP targets PELI2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PELI2 fusion protein Ag8940 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pellino homolog 2 (Drosophila)
别名 RING-type E3 ubiquitin transferase pellino homolog 2, Protein pellino homolog 2, Pellino-2, Pellino 2, EC:2.3.2.27
计算分子量 420 aa, 46 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC009476
基因名称 PELI2
Gene ID (NCBI) 57161
RRIDAB_2878218
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pellino2 (PELI2) is a newly discovered E3 ubiquitin ligase, which regulates the protein degradation, protein-protein interaction, protein translocation and signaling transduction via the ubiquitination of target proteins, and plays important roles in inflammation and the immune system. PELI2 possesses a C-terminal RING-like domain and a phospho-threonine-binding forkhead-associated (FHA) domain that are responsible for ubiquitin ligase activity and substrate binding, respectively (PMID: 24699078).

实验方案

Product Specific Protocols
WB protocol for PELI2 antibody 16097-1-APDownload protocol
IHC protocol for PELI2 antibody 16097-1-APDownload protocol
IF protocol for PELI2 antibody 16097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols