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验证数据展示

  • WB analysis using 87624-2-RR (same clone as 87624-2-PBS)

    WB analysis using 87624-2-RR (same clone as 87624-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87624-2-RR (PELI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87624-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 87624-2-RR (same clone as 87624-2-PBS)

    IP experiment of mouse brain using 87624-2-RR (same clone as 87624-2-PBS)

    IP result of anti-PELI1 (IP:87624-2-RR, 4ug; Detection:87624-2-RR 1:500) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 87624-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 87624-2-RR (same clone as 87624-2-PBS)

    IHC staining of mouse liver using 87624-2-RR (same clone as 87624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 87624-2-RR (PELI1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87624-2-PBS in a different storage buffer formulation.

  • Affinity of 87624-2-RR

    Affinity of 87624-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87624-2-RR against Human PELI1 were performed. The affinity constant is below 1 pM.

PELI1 Recombinant monoclonal antibody, PBS Only

PELI1 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 87624-2-PBS

产品说明书

CloneNo. 252984C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

E3 ubiquitin-protein ligase pellino homolog 1, EC:2.3.2.27, Pellino 1, Pellino-1, Pellino-related intracellular-signaling molecule

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87624-2-PBS targets PELI1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2683

Product name: Recombinant human PELI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 294-418 aa of BC011419

Sequence: MKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pellino homolog 1 (Drosophila)
别名 E3 ubiquitin-protein ligase pellino homolog 1, EC:2.3.2.27, Pellino 1, Pellino-1, Pellino-related intracellular-signaling molecule
计算分子量 418 aa, 46 kDa
观测分子量 46-53 kDa
GenBank蛋白编号BC011419
基因名称 PELI1
Gene ID (NCBI) 57162
RRIDAB_3745658
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96FA3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PELI1 encodes pellino-1, an E3 ubiquitin-protein ligase pellino homolog. E3 ubiquitin ligase functions by catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Pellino-1 is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Pellino-1 can mediate 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation thus playing a role in cellular inflammation.

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