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PEG3 Polyclonal antibody

PEG3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23569-1-AP

Print datasheet

Synonyms

KIAA0287, paternally expressed 3, PEG3, PW1, ZSCAN24

验证数据展示

  • WB analysis using 23569-1-AP

    WB analysis using 23569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23569-1-AP (PEG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 23569-1-AP

    IHC staining of human placenta using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 23569-1-AP

    IHC staining of mouse brain using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 23569-1-AP

    IHC staining of human placenta using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, TT cells
Positive IHC detected inhuman placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23569-1-AP targets PEG3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen PEG3 fusion protein Ag20132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name paternally expressed 3
Synonyms KIAA0287, paternally expressed 3, PEG3, PW1, ZSCAN24
Calculated Molecular Weight 1588 aa, 181 kDa
Observed Molecular Weight200-220 kDa
GenBank Accession NumberBC150272
Gene Symbol PEG3
Gene ID (NCBI) 5178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9GZU2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).

实验方案

Product Specific Protocols
WB protocol for PEG3 antibody 23569-1-APDownload protocol
IHC protocol for PEG3 antibody 23569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols