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验证数据展示

  • WB analysis using 86928-4-RR (same clone as 86928-4-PBS)

    WB analysis using 86928-4-RR (same clone as 86928-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86928-4-RR (PEG10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86928-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86928-4-RR

    Affinity and Kinetic Characterization of 86928-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86928-4-RR against Human PEG10 were performed. The affinity constant is 2.61 nM.

PEG10 Recombinant monoclonal antibody, PBS Only

PEG10 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86928-4-PBS

产品说明书

CloneNo. 251980A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

EDR, Embryonal carcinoma differentiation-regulated protein, HB 1, HB1, KIAA1051

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PEG10 Ready-To-Use IHC Kit. PEG10 primary antibody included.

产品信息

86928-4-PBS targets PEG10 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5754

Product name: Recombinant human PEG10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-325 aa of BC050659

Sequence: MTERRRDELSEEINNLREKVMKQSEENNNLQSQVQKLTEENTTLREQVEPTPEDEDDDIELRGAAAAAAPPPPIEEECPEDLPEKFDGNPDMLAPFMAQCQIFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMMEMKHVFEDPQRREVAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQEELSHLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALVLPHIASHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGTGGHYADNCPAKASKSSPAGNSPAPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 paternally expressed 10
别名 EDR, Embryonal carcinoma differentiation-regulated protein, HB 1, HB1, KIAA1051
计算分子量 80 kDa
观测分子量 50 kDa, 100 kDa
GenBank蛋白编号BC050659
基因名称 PEG10
Gene ID (NCBI) 23089
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ86TG7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Paternally expressed imprinted gene 10 (PEG10) is an evolutionarily conserved HERV gene belonging to the Ty3/Gypsy family of retrotransposons. The mRNA of PEG10 encodes at least two protein isoforms: the major PEG10 protein product reading frame 1 (RF1-PEG10, ~50 kDa) and reading frames 1 and 2 (RF1/RF2-PEG10, ~100 kDa), which are translated by a typical retroviral-1 ribosomal frameshift mechanism. PEG10 is strongly expressed in placenta, ovary and testis as well as somatic adult organs including brain, kidney and lung . Researches on mice suggest that PEG10 plays an important role in placenta formation and adipocyte differentiation, and its knockout can cause embryonic lethality.(PMID: 30123090, PMID: 32244497)

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