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验证数据展示

  • WB analysis using 68278-1-Ig

    WB analysis using 68278-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68278-1-Ig (PDXDC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of MCF-7 using 68278-1-Ig

    IF Staining of MCF-7 using 68278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PDXDC1 antibody (68278-1-Ig, Clone: 1E6E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of MCF-7 using 68278-1-Ig

    IF Staining of MCF-7 using 68278-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDXDC1 antibody (68278-1-Ig, Clone: 1E6E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 68278-1-Ig

    WB analysis using 68278-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68278-1-Ig (PDXDC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

PDXDC1 Monoclonal antibody

PDXDC1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68278-1-Ig

产品说明书

CloneNo. 1E6E2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

KIAA0251, LP8165, PDXDC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells, pig colon tissue, rat colon tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68278-1-Ig targets PDXDC1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 PDXDC1 fusion protein Ag15489 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pyridoxal-dependent decarboxylase domain containing 1
别名 KIAA0251, LP8165, PDXDC1
计算分子量 788 aa, 87 kDa
观测分子量87 kDa
GenBank蛋白编号BC033748
基因名称 PDXDC1
Gene ID (NCBI) 23042
RRIDAB_2935359
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6P996
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDXDC1 (pyridoxal-dependent decarboxylase domain containing 1) is a putative enzyme that could metabolize catecholamine neurotransmitters (PMID:28485732). With prior evidence for involvement with glioblastoma from other previously reported experimental settings, and contains the lead single nucleotide polymorphism (rs3198697) from the linkage analysis of the chromosome 16 region (PMID:32644145). Moreover, PDXDC1 is involved in the catalysis of the nonhydrolytic addition or removal of a carboxyl group to or from a compound (PMID:29277971).

实验方案

Product Specific Protocols
WB protocol for PDXDC1 antibody 68278-1-IgDownload protocol
IF protocol for PDXDC1 antibody 68278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols