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Podoplanin Polyclonal antibody

Podoplanin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22099-1-AP

Print datasheet

Synonyms

AGGRUS, D2 40, D2 40,M2A, D240, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, Podoplanin, PSEC0025, T1 alpha, T1A, T1A 2

验证数据展示

  • WB analysis of human brain using 22099-1-AP

    WB analysis of human brain using 22099-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 22099-1-AP

    IHC staining of human appendicitis using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 22099-1-AP

    IHC staining of human appendicitis using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, human placenta tissue
Positive IHC detected inhuman appendicitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22099-1-AP is Podoplanin Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen Podoplanin fusion protein Ag17307 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name podoplanin
Synonyms AGGRUS, D2 40, D2 40,M2A, D240, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, Podoplanin, PSEC0025, T1 alpha, T1A, T1A 2
Calculated Molecular Weight 238 aa, 25 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC022812
Gene Symbol PDPN
Gene ID (NCBI) 10630
RRIDAB_2878995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Podoplanin antibody 22099-1-APDownload protocol
IHC protocol for Podoplanin antibody 22099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols