• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86283-1-RR

    WB analysis using 86283-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86283-1-RR (PDLIM7,LMP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of SKOV-3 using 86283-1-RR

    IF Staining of SKOV-3 using 86283-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDLIM7 antibody (86283-1-RR, Clone: 250775F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86283-1-RR

    Affinity and Kinetic Characterization of 86283-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86283-1-RR against Human PDLIM7 were performed. The affinity constant is 8.36 nM.

PDLIM7 Recombinant monoclonal antibody

PDLIM7 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86283-1-RR

产品说明书

CloneNo. 250775F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ENIGMA, LIM mineralization protein, LMP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, SKOV-3 cells, K-562 cells
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy PDLIM7 Ready-To-Use IHC Kit. PDLIM7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86283-1-RR targets PDLIM7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4614

Product name: Recombinant human PDLIM7,LMP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 3-289 aa of BC001093

Sequence: SFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PDZ and LIM domain 7 (enigma)
别名 ENIGMA, LIM mineralization protein, LMP1
计算分子量 55/60 kDa
观测分子量 50 kDa
GenBank蛋白编号BC001093
基因名称 PDLIM7
Gene ID (NCBI) 9260
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NR12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

实验方案

Product Specific Protocols
IF protocol for PDLIM7 antibody 86283-1-RRDownload protocol
WB protocol for PDLIM7 antibody 86283-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...