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验证数据展示

  • WB analysis using 86283-1-RR (same clone as 86283-1-PBS)

    WB analysis using 86283-1-RR (same clone as 86283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86283-1-RR (PDLIM7,LMP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86283-1-PBS in a different storage buffer formulation.

  • IF Staining of SKOV-3 using 86283-1-RR (same clone as 86283-1-PBS)

    IF Staining of SKOV-3 using 86283-1-RR (same clone as 86283-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDLIM7 antibody (86283-1-RR, Clone: 250775F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86283-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86283-1-RR

    Affinity and Kinetic Characterization of 86283-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86283-1-RR against Human PDLIM7 were performed. The affinity constant is 8.36 nM.

PDLIM7 Recombinant monoclonal antibody, PBS Only

PDLIM7 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86283-1-PBS

产品说明书

CloneNo. 250775F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ENIGMA, LIM mineralization protein, LMP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDLIM7 Ready-To-Use IHC Kit. PDLIM7 primary antibody included.

产品信息

86283-1-PBS targets PDLIM7 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4614

Product name: Recombinant human PDLIM7,LMP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 3-289 aa of BC001093

Sequence: SFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PDZ and LIM domain 7 (enigma)
别名 ENIGMA, LIM mineralization protein, LMP1
计算分子量 55/60 kDa
观测分子量 50 kDa
GenBank蛋白编号BC001093
基因名称 PDLIM7
Gene ID (NCBI) 9260
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NR12
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

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