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  • Featured Product
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PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17080-1-AP

Print datasheet

Synonyms

ENIGMA, LIM mineralization protein, LMP, LMP1, PDLIM7, PDZ and LIM domain 7 (enigma), PDZ and LIM domain protein 7, Protein enigma

验证数据展示

  • WB analysis of HeLa using 17080-1-AP

    WB analysis of HeLa using 17080-1-AP

    WB result of PDLIM7 antibody (17080-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

  • WB analysis using 17080-1-AP

    WB analysis using 17080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17080-1-AP (PDLIM7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17080-1-AP

    IHC staining of human liver using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PDLIM7 antibody included
  • IHC staining of human liver using 17080-1-AP

    IHC staining of human liver using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PDLIM7 antibody included
  • IP experiment of Jurkat using 17080-1-AP

    IP experiment of Jurkat using 17080-1-AP

    IP result of anti-PDLIM7 (IP:17080-1-AP, 4ug; Detection:17080-1-AP 1:1000) with Jurkat cells lysate 3600ug.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue, human kidney tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDLIM7 Ready-To-Use IHC Kit. PDLIM7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17080-1-AP targets PDLIM7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen PDLIM7 fusion protein Ag1334 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDZ and LIM domain 7 (enigma)
Synonyms ENIGMA, LIM mineralization protein, LMP, LMP1, PDLIM7, PDZ and LIM domain 7 (enigma), PDZ and LIM domain protein 7, Protein enigma
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC014521
Gene Symbol PDLIM7
Gene ID (NCBI) 9260
RRIDAB_2161788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDLIM7 antibody 17080-1-APDownload protocol
IHC protocol for PDLIM7 antibody 17080-1-APDownload protocol
IP protocol for PDLIM7 antibody 17080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols