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验证数据展示

  • WB analysis of HeLa using 17080-1-AP

    WB analysis of HeLa using 17080-1-AP

    WB result of PDLIM7 antibody (17080-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

  • WB analysis using 17080-1-AP

    WB analysis using 17080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17080-1-AP (PDLIM7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 17080-1-AP

    IHC staining of mouse skeletal muscle using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDLIM7 antibody included
  • IP experiment of HeLa using 17080-1-AP

    IP experiment of HeLa using 17080-1-AP

    IP result of anti-PDLIM7 (IP:17080-1-AP, 4ug; Detection:17080-1-AP 1:5000) with HeLa cells lysate 1200 ug.

  • IHC staining of human colon using 17080-1-AP

    IHC staining of human colon using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDLIM7 antibody included
  • IHC staining of mouse skeletal muscle using 17080-1-AP

    IHC staining of mouse skeletal muscle using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDLIM7 antibody included
  • Featured Product
  • KD/KO验证

PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17080-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

ENIGMA, LIM mineralization protein, LMP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue, human colon, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDLIM7 Ready-To-Use IHC Kit. PDLIM7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17080-1-AP targets PDLIM7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 PDLIM7 fusion protein Ag1334 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ and LIM domain 7 (enigma)
别名 ENIGMA, LIM mineralization protein, LMP1
计算分子量 50 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC014521
基因名称 PDLIM7
Gene ID (NCBI) 9260
RRIDAB_2161788
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDLIM7 antibody 17080-1-APDownload protocol
IHC protocol for PDLIM7 antibody 17080-1-APDownload protocol
IP protocol for PDLIM7 antibody 17080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols