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验证数据展示

  • WB analysis using 18543-1-AP (same clone as 18543-1-PBS)

    WB analysis using 18543-1-AP (same clone as 18543-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18543-1-AP (PDLIM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 18543-1-AP (same clone as 18543-1-PBS)

    WB analysis of A431 using 18543-1-AP (same clone as 18543-1-PBS)

    WB result of PDLIM4 antibody (18543-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM4 transfected A431 cells. This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 18543-1-AP (same clone as 18543-1-PBS)

    IHC staining of human skin cancer using 18543-1-AP (same clone as 18543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18543-1-AP (PDLIM4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDLIM4 antibody included
  • IF Staining of U2OS using 18543-1-AP (same clone as 18543-1-PBS)

    IF Staining of U2OS using 18543-1-AP (same clone as 18543-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PDLIM4 antibody (18543-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PDLIM4 Polyclonal antibody, PBS Only

PDLIM4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18543-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PDZ and LIM domain 4, Reversion-induced LIM protein, RIL

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDLIM4 Ready-To-Use IHC Kit. PDLIM4 primary antibody included.

产品信息

18543-1-PBS targets PDLIM4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13424

Product name: Recombinant human PDLIM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC016765

Sequence: MPHSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPEIQDGSPTTSRGPSGTGTGPEDGRPSLGSPYGQPPRFPVPHNGSSEATLPAQMSTLHVSPPPSADPARGLPRSRDCRVDLGSEVYRMLREPAEPVAAEPKQSGSFRYLQGMLEAGEGGDWPGPGGPRNLKPTASKLGAPLSGLQGLPECTRCGHGIVGTIVKARDKLYHPECFMCSDCGLNLKQRGYFFLDERLYCESHAKARVKPPEGYDVVAVYPNAKVELV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ and LIM domain 4
别名 PDZ and LIM domain 4, Reversion-induced LIM protein, RIL
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC016765
基因名称 PDLIM4
Gene ID (NCBI) 8572
RRIDAB_3085565
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50479
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099).

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