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PDLIM4 Polyclonal antibody

PDLIM4 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 18543-1-AP

Print datasheet

Synonyms

LIM protein RIL, PDLIM4, PDZ and LIM domain 4, PDZ and LIM domain protein 4, Reversion induced LIM protein, RIL

验证数据展示

  • WB analysis using 18543-1-AP

    WB analysis using 18543-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18543-1-AP (PDLIM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 18543-1-AP

    IF Staining of U2OS using 18543-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PDLIM4 antibody (18543-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, K-562 cells
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18543-1-AP targets PDLIM4 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen PDLIM4 fusion protein Ag13424 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDZ and LIM domain 4
Synonyms LIM protein RIL, PDLIM4, PDZ and LIM domain 4, PDZ and LIM domain protein 4, Reversion induced LIM protein, RIL
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC016765
Gene Symbol PDLIM4
Gene ID (NCBI) 8572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50479
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099).

实验方案

Product Specific Protocols
WB protocol for PDLIM4 antibody 18543-1-APDownload protocol
IF protocol for PDLIM4 antibody 18543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols