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验证数据展示

  • WB analysis using 12949-1-AP (same clone as 12949-1-PBS)

    WB analysis using 12949-1-AP (same clone as 12949-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

    IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDK4 antibody included
  • IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

    IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDK4 antibody included
  • Featured Product
  • KD/KO Validated

PDK4 Polyclonal antibody, PBS Only

PDK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12949-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, EC:2.7.11.2, PDHK4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDK4 Ready-To-Use IHC Kit. PDK4 primary antibody included.

产品信息

12949-1-PBS targets PDK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3629

Product name: Recombinant human PDK4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-412 aa of BC040239

Sequence: LPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyruvate dehydrogenase kinase, isozyme 4
别名 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, EC:2.7.11.2, PDHK4
计算分子量 411 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC040239
基因名称 PDK4
Gene ID (NCBI) 5166
RRIDAB_2161499
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16654
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).

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