• Featured Product
  • KD/KO验证

PDK4 Polyclonal antibody

PDK4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12949-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF/ICC, ELISA and More (1)

PDHK4, EC:2.7.11.2, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, NCI-H1299 cells, mouse heart tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDK4 Ready-To-Use IHC Kit. PDK4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12949-1-AP targets PDK4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, bovine, goat
免疫原 PDK4 fusion protein Ag3629 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyruvate dehydrogenase kinase, isozyme 4
别名 PDHK4, EC:2.7.11.2, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
计算分子量 411 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC040239
基因名称 PDK4
Gene ID (NCBI) 5166
RRIDAB_2161499
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16654
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).

实验方案

Product Specific Protocols
WB protocol for PDK4 antibody 12949-1-APDownload protocol
IHC protocol for PDK4 antibody 12949-1-APDownload protocol
IF protocol for PDK4 antibody 12949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine

Authors - Mengqi Li
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
humanWB

Sci Transl Med

Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.

Authors - Biying Zhu
humanWB

Mol Syst Biol

STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.

Authors - Monika Oberhuber
humanWB

Theranostics

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Authors - Xiaoxin Zhang
mouseIF

Diabetes

Glucose controls glucagon secretion by regulating fatty acid oxidation in pancreatic alpha cells

Authors - Sarah L Armour
Loading...