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验证数据展示

  • WB analysis using 85599-4-RR (same clone as 85599-4-PBS)

    WB analysis using 85599-4-RR (same clone as 85599-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85599-4-RR (PDIA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85599-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 85599-4-RR (same clone as 85599-4-PBS)

    IHC staining of human placenta using 85599-4-RR (same clone as 85599-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85599-4-RR (PDIR antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85599-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 85599-4-RR (same clone as 85599-4-PBS)

    IHC staining of human placenta using 85599-4-RR (same clone as 85599-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85599-4-RR (PDIR antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85599-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85599-4-RR

    Affinity and Kinetic Characterization of 85599-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85599-4-RR against Human PDIR were performed. The affinity constant is below 1 pM.

PDIR Recombinant monoclonal antibody, PBS Only

PDIR Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85599-4-PBS

产品说明书

CloneNo. 242971D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

PDIA5, EC:5.3.4.1, Protein disulfide isomerase A5, Protein disulfide isomerase-related protein, Protein disulfide-isomerase A5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85599-4-PBS targets PDIR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31479

Product name: Recombinant human PDIA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-170 aa of BC001625

Sequence: SSAKVSSLIERISDPKDLKKLLRTRNNVLVLYSKSEVAAENHLRLLSTVAQAVKGQGTICWVDCGDAESRKLCKKMKVDLSPKDKKVELFHYQDGAFHTEYNRAVTFKSIVAFLKDPKGPPLWEEDPGAKDVVHLDSEKDFRRLLKKEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein disulfide isomerase family A, member 5
别名 PDIA5, EC:5.3.4.1, Protein disulfide isomerase A5, Protein disulfide isomerase-related protein, Protein disulfide-isomerase A5
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC001625
基因名称 PDIR
Gene ID (NCBI) 10954
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14554
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIR(Protein disulfide isomerase-related protein), also known as PDIA5 (Protein Disulfide Isomerase Family A Member 5), is a member of the PDI gene family and also exhibits chaperone-like activity. PDIR protein is correlated with immune infiltration, which is highly expressed in glioma and participates in glioma progression(PMID: 33664747). PDIR contributes to disulfide bond rearrangement in ATF6α under stress conditions that necessary for ATF6α activation upon ER stress(PMID: 24636989).

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