验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HeLa, HepG2, HEK-293, L02, K-562, NIH-3T3, HSC-T6 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87234-1-RR targets PDIA6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Eg5454 Product name: Recombinant Human PDIA6 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-440 aa of NM_005742.3 Sequence: LYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDEL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein disulfide isomerase family A, member 6 |
| 别名 | EC:5.3.4.1, ER protein 5, ERp5, P5, Protein disulfide isomerase A6 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | NM_005742.3 |
| 基因名称 | PDIA6 |
| Gene ID (NCBI) | 10130 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15084-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PDIA6 antibody 87234-1-RR | Download protocol |
| WB protocol for PDIA6 antibody 87234-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
