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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 87234-1-RR (same clone as 87234-1-PBS)

    WB analysis using 87234-1-RR (same clone as 87234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87234-1-RR (PDIA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87234-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87234-1-RR (same clone as 87234-1-PBS)

    IF Staining of HeLa using 87234-1-RR (same clone as 87234-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDIA6 antibody (87234-1-RR, Clone: 252495E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87234-1-PBS in a different storage buffer formulation.

  • Affinity of 87234-1-RR

    Affinity of 87234-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87234-1-RR against Human PDIA6 were performed. The affinity constant is 1.71 nM.

PDIA6 Recombinant monoclonal antibody, PBS Only

PDIA6 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87234-1-PBS

产品说明书

CloneNo. 252495E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

EC:5.3.4.1, ER protein 5, ERp5, P5, Protein disulfide isomerase A6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDIA6 Ready-To-Use IHC Kit. PDIA6 primary antibody included.

产品信息

87234-1-PBS targets PDIA6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg5454

Product name: Recombinant Human PDIA6 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-440 aa of NM_005742.3

Sequence: LYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein disulfide isomerase family A, member 6
别名 EC:5.3.4.1, ER protein 5, ERp5, P5, Protein disulfide isomerase A6
计算分子量48 kDa
观测分子量54 kDa
GenBank蛋白编号NM_005742.3
基因名称 PDIA6
Gene ID (NCBI) 10130
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15084-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.

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