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验证数据展示

  • WB analysis using 86239-1-RR (same clone as 86239-1-PBS)

    WB analysis using 86239-1-RR (same clone as 86239-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86239-1-RR (PDIA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86239-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86239-1-RR (same clone as 86239-1-PBS)

    IF Staining of HeLa using 86239-1-RR (same clone as 86239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDIA4 antibody (86239-1-RR, Clone: 250697E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86239-1-PBS in a different storage buffer formulation.

PDIA4 Recombinant monoclonal antibody, PBS Only

PDIA4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86239-1-PBS

产品说明书

CloneNo. 250697E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

ERp72, EC:5.3.4.1, ERp 72, ERp70, ERp-72

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDIA4/ERP72 Ready-To-Use IHC Kit. PDIA4/ERP72 primary antibody included.

产品信息

86239-1-PBS targets PDIA4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg3011

Product name: Recombinant Human PDIA4/ERp72 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 21-641 aa of NM_004911.5

Sequence: VAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein disulfide isomerase family A, member 4
别名 ERp72, EC:5.3.4.1, ERp 72, ERp70, ERp-72
计算分子量73 kDa
观测分子量75 kDa
GenBank蛋白编号NM_004911.5
基因名称 ERp72
Gene ID (NCBI) 9601
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13667
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

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