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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80170-1-RR (same clone as 80170-1-PBS)

    WB analysis using 80170-1-RR (same clone as 80170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80170-1-RR (PDI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 80170-1-RR (same clone as 80170-1-PBS)

    IHC staining of human prostate cancer using 80170-1-RR (same clone as 80170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDI/P4HB antibody included
  • WB analysis of HEK-293 using 80170-1-RR (same clone as 80170-1-PBS)

    WB analysis of HEK-293 using 80170-1-RR (same clone as 80170-1-PBS)

    WB result of PDI antibody (80170-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells. This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse lung using 80170-1-RR (same clone as 80170-1-PBS)

    IF Staining of mouse lung using 80170-1-RR (same clone as 80170-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using PDI antibody (80170-1-RR, Clone: 4K17 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80170-1-RR

    Affinity and Kinetic Characterization of 80170-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80170-1-RR against Human PDI were performed. The affinity constant is 13.8 nM.

  • Featured Product
  • KD/KO验证

PDI Recombinant antibody, PBS Only

PDI Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 80170-1-PBS

产品说明书

CloneNo. 4K17

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

P4HB, 4K17, Cellular thyroid hormone-binding protein, CLCRP1, DSI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.

产品信息

80170-1-PBS targets PDI in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PDI fusion protein Ag1747 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prolyl 4-hydroxylase, beta polypeptide
别名 P4HB, 4K17, Cellular thyroid hormone-binding protein, CLCRP1, DSI
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC014504
基因名称 PDI
Gene ID (NCBI) 5034
RRIDAB_2918871
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07237
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).