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验证数据展示

  • WB analysis using 82943-1-RR

    WB analysis using 82943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82943-1-RR

    WB analysis using 82943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 82943-1-RR

    IHC staining of human placenta using 82943-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDGFRB antibody included
  • IHC staining of human placenta using 82943-1-RR

    IHC staining of human placenta using 82943-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDGFRB antibody included
  • Affinity and Kinetic Characterization of 82943-1-RR

    Affinity and Kinetic Characterization of 82943-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82943-1-RR against Human PDGFRB were performed. The affinity constant is 0.668 nM.

PDGFR beta/CD140b Recombinant antibody

PDGFR beta/CD140b Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82943-1-RR

产品说明书

CloneNo. 230214D11
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

PDGFRB, EC:2.7.10.1, CD140B, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMG-63 cells, HeLa cells, COLO 320 cells, U-87 MG cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDGFRB Ready-To-Use IHC Kit. PDGFRB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

82943-1-RR targets PDGFR beta/CD140b in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 platelet-derived growth factor receptor, beta polypeptide
别名 PDGFRB, EC:2.7.10.1, CD140B, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor
计算分子量 1106 aa, 124 kDa
观测分子量180 kDa
GenBank蛋白编号BC032224
基因名称 PDGFR beta
Gene ID (NCBI) 5159
RRIDAB_3670686
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDGFR beta/CD140b antibody 82943-1-RRDownload protocol
IHC protocol for PDGFR beta/CD140b antibody 82943-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Acta Diabetol

The UBR5 protein facilitates mesangial cell hypertrophy and glycolysis induced by high glucose by increasing the phosphorylation levels of AKT

Authors - Lin Liao
View Article