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验证数据展示

  • WB analysis of rat lung using 87506-1-RR (same clone as 87506-1-PBS)

    WB analysis of rat lung using 87506-1-RR (same clone as 87506-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 87506-1-RR (PDGF-BB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87506-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 87506-1-RR (same clone as 87506-1-PBS)

    WB analysis of SH-SY5Y using 87506-1-RR (same clone as 87506-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 87506-1-RR (PDGF-BB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87506-1-PBS in a different storage buffer formulation.

  • WB analysis using 87506-1-RR (same clone as 87506-1-PBS)

    WB analysis using 87506-1-RR (same clone as 87506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87506-1-RR (PDGF-BB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87506-1-PBS in a different storage buffer formulation.

  • Affinity of 87506-1-RR

    Affinity of 87506-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87506-1-RR against Mouse PDGF-BB were performed. The affinity constant is 2.21 nM.

Mouse PDGF-BB Recombinant monoclonal antibody, PBS Only

PDGF-BB Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87506-1-PBS

产品说明书

CloneNo. 252712C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

PDGF subunit B, PDGF-2, Pdgfb, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87506-1-PBS targets PDGF-BB in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg4856

Product name: recombinant mouse PDGF-BB protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 82-190 aa of AAH23427.1

Sequence: SLGSLAAAEPAVIAECKTRTEVFQISRNLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRASQVQMRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETIVTPRPVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 platelet derived growth factor, B polypeptide
别名 PDGF subunit B, PDGF-2, Pdgfb, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide
计算分子量25kd
观测分子量30 kDa
GenBank蛋白编号AAH23427.1
基因名称 Pdgfb
Gene ID (NCBI) 18591
RRIDAB_3745588
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31240
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.

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