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PDE8B Polyclonal antibody

PDE8B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 30708-1-AP

Print datasheet

Synonyms

FLJ11212, HsPDE8B, PDE8B, phosphodiesterase 8B, PPNAD3

验证数据展示

  • WB analysis using 30708-1-AP

    WB analysis using 30708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30708-1-AP (PDE8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30708-1-AP

    IHC staining of mouse testis using 30708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30708-1-AP (PDE8B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30708-1-AP

    IHC staining of mouse testis using 30708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30708-1-AP (PDE8B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human placenta tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30708-1-AP targets PDE8B in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA
Tested Reactivity Human, mouse
Immunogen PDE8B fusion protein Ag33477 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphodiesterase 8B
Synonyms FLJ11212, HsPDE8B, PDE8B, phosphodiesterase 8B, PPNAD3
Calculated Molecular Weight99kd
Observed Molecular Weight68-98 kDa
GenBank Accession NumberNM_003719
Gene Symbol PDE8B
Gene ID (NCBI) 8622
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human phosphodiesterase (PDE) type 8B (PDE8B) is located at 5q14.1 and is known as the PDE with the highest affinity to cAMP.

实验方案

Product Specific Protocols
WB protocol for PDE8B antibody 30708-1-APDownload protocol
IHC protocol for PDE8B antibody 30708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols