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验证数据展示

  • WB analysis using 30708-1-AP

    WB analysis using 30708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30708-1-AP (PDE8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30708-1-AP

    IHC staining of mouse testis using 30708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30708-1-AP (PDE8B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30708-1-AP

    IHC staining of mouse testis using 30708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30708-1-AP (PDE8B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 30708-1-AP

    IF Staining of mouse testis using 30708-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PDE8B antibody (30708-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PDE8B Polyclonal antibody

PDE8B Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 30708-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF-P, ELISA

别名

HsPDE8B, phosphodiesterase 8B, PPNAD3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human placenta tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

30708-1-AP targets PDE8B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, sheep
免疫原

CatNo: Ag33477

Product name: Recombinant human PDE8B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 374-440 aa of NM_003719

Sequence: FVSLKKLCCTTDNNKQIHKIHRDSGDNSQTEPHSFRYKNRRKESIDVKSISSRGSDAPSLQNRRYPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphodiesterase 8B
别名 HsPDE8B, phosphodiesterase 8B, PPNAD3
计算分子量99kd
观测分子量68-98 kDa
GenBank蛋白编号NM_003719
基因名称 PDE8B
Gene ID (NCBI) 8622
RRIDAB_3086395
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95263
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human phosphodiesterase (PDE) type 8B (PDE8B) is located at 5q14.1 and is known as the PDE with the highest affinity to cAMP.

实验方案

Product Specific Protocols
IF protocol for PDE8B antibody 30708-1-APDownload protocol
IHC protocol for PDE8B antibody 30708-1-APDownload protocol
WB protocol for PDE8B antibody 30708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cardiovasc Med

Machine learning-driven exploration of therapeutic targets for atrial fibrillation-joint analysis of single-cell and bulk transcriptomes and experimental validation.

Authors - Yicheng Wang
View Article
sheepWB

Animals (Basel)

DIA Proteomics Reveals the Mechanism of cAMP Signaling Pathway-Mediated HPT Axis in Regulating Spermatogenesis of Hu Sheep.

Authors - Lina Zhu
View Article
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