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验证数据展示

  • WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

    WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

    IP experiment of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

    IP result of anti-PDE8A (IP:13956-1-AP, 5ug; Detection:13956-1-AP 1:1000) with mouse testis tissue lysate 8000ug. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

    IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDE8A antibody included
  • IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

    IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDE8A antibody included

PDE8A Polyclonal antibody, PBS Only

PDE8A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13956-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDE8A Ready-To-Use IHC Kit. PDE8A primary antibody included.

产品信息

13956-1-PBS targets PDE8A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5069

Product name: Recombinant human PDE8A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 530-829 aa of BC060762

Sequence: FLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLHATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVNSINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEWAARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphodiesterase 8A
别名
计算分子量 93 kDa
观测分子量 93 kDa, 88 kDa
GenBank蛋白编号BC060762
基因名称 PDE8A
Gene ID (NCBI) 5151
RRIDAB_2161728
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60658
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832).

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