验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, T-47D cells, mouse brain tissue, rat brain tissue, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83503-1-RR targets PDE6D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PDE6D fusion protein Ag29563 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | phosphodiesterase 6D, cGMP-specific, rod, delta |
| 别名 | PDED, 230546H1 |
| 计算分子量 | 17 KDa |
| 观测分子量 | 14-17 kDa |
| GenBank蛋白编号 | NM_002601 |
| 基因名称 | PDE6D |
| Gene ID (NCBI) | 5147 |
| RRID | AB_3671128 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O43924 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE6D antibody 83503-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |