验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, human placenta tissue, rat retina tissue, mouse brain tissue, JAR cells, Pig brain tissue, Rat brain tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
67565-1-Ig targets PDE6D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag29563 Product name: Recombinant human PDE6D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of NM_002601 Sequence: MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphodiesterase 6D, cGMP-specific, rod, delta |
| 别名 | 1H4B6, PDED |
| 计算分子量 | 17 KDa |
| 观测分子量 | 15-17 kDa |
| GenBank蛋白编号 | NM_002601 |
| 基因名称 | PDE6D |
| Gene ID (NCBI) | 5147 |
| RRID | AB_2882779 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43924 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDE6D antibody 67565-1-Ig | Download protocol |
| WB protocol for PDE6D antibody 67565-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
