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验证数据展示

  • WB analysis of Jurkat using 67565-1-Ig

    WB analysis of Jurkat using 67565-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67565-1-Ig

    WB analysis using 67565-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67565-1-Ig

    IHC staining of mouse brain using 67565-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDE6D Monoclonal antibody

PDE6D Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67565-1-Ig

产品说明书

CloneNo. 1H4B6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

PDED, 1H4B6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, human placenta tissue, rat retina tissue, mouse brain tissue, JAR cells, Pig brain tissue, Rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67565-1-Ig targets PDE6D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PDE6D fusion protein Ag29563 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphodiesterase 6D, cGMP-specific, rod, delta
别名 PDED, 1H4B6
计算分子量 17 KDa
观测分子量 15-17 kDa
GenBank蛋白编号NM_002601
基因名称 PDE6D
Gene ID (NCBI) 5147
RRIDAB_2882779
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43924
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

实验方案

Product Specific Protocols
WB protocol for PDE6D antibody 67565-1-IgDownload protocol
IHC protocol for PDE6D antibody 67565-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols