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验证数据展示

  • WB analysis of human heart using 13785-1-AP (same clone as 13785-1-PBS)

    WB analysis of human heart using 13785-1-AP (same clone as 13785-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13785-1-AP (PDE1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13785-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 13785-1-AP (same clone as 13785-1-PBS)

    IP experiment of mouse heart using 13785-1-AP (same clone as 13785-1-PBS)

    IP result of anti-PDE1C (IP:13785-1-AP, 4ug; Detection:13785-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 13785-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 13785-1-AP (same clone as 13785-1-PBS)

    IHC staining of human liver using 13785-1-AP (same clone as 13785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13785-1-AP (PDE1C antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13785-1-PBS in a different storage buffer formulation.

PDE1C Polyclonal antibody, PBS Only

PDE1C Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13785-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

Cam PDE 1C, Cam-PDE 1C, Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C, EC:3.1.4.17, hCam 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13785-1-PBS targets PDE1C in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4798

Product name: Recombinant human PDE1C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 284-634 aa of BC022525

Sequence: RSDPAILYNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKAMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISVDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARMAAEEQQKEMEAKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphodiesterase 1C, calmodulin-dependent 70kDa
别名 Cam PDE 1C, Cam-PDE 1C, Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C, EC:3.1.4.17, hCam 3
计算分子量 634 aa, 72 kDa
观测分子量 68 kDa
GenBank蛋白编号BC022525
基因名称 PDE1C
Gene ID (NCBI) 5137
RRIDAB_2268145
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14123
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1C, is calmodulin-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex. Expression of PDE1C may be an important component in signal transduction pathways mediated by calcium that permit cell cycle traverse and proliferation(PMID:9366577). It has 2 isoforms produced by alternative splicing.

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