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CoraLite® Plus 488-conjugated PDCD4 Monoclonal antibody
PDCD4 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F1A11
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 66100-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66100 targets PDCD4 in WB applications and shows reactivity with human samples.
| Tested Applications | WB |
| Tested Reactivity | human |
| Immunogen | PDCD4 fusion protein Ag19239 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | programmed cell death 4 (neoplastic transformation inhibitor) |
| Synonyms | H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a |
| Calculated Molecular Weight | 469 aa, 52 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC026104 |
| Gene Symbol | PDCD4 |
| Gene ID (NCBI) | 27250 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q53EL6 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 PDCD4 antibody CL488-66100 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |