Sandwich ELISA standard curve of MP00601-4, Human PD-L1/CD274 Recombinant Matched Antibody Pair - PBS only. 83600-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1114. 83600-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean PD-L1/CD274 concentration was determined to be 7.7 pg/mL in unstimulated PBMC supernatant, 108.4 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean PD-L1/CD274 concentration was determined to be 11.0 pg/mL in unstimulated PBMC supernatant, 160.7 pg/mL in PHA stimulated PBMC supernatant.

Urine of seven individual healthy human donors was measured. The PD-L1/CD274 concentration of detected samples was determined to be 10.8 pg/mL with a range of 0.7 - 33.1 pg/mL.

The mean PD-L1/CD274 concentration was determined to be 36,368.3 pg/mL in MDA-MB-231 cell extract based on a 2.3 mg/mL extract load.

Serum of sixteen individual healthy human donors was measured. The PD-L1/CD274 concentration of detected samples was determined to be 75.1 pg/mL with a range of 34.4-168.1 pg/mL.

Biolayer interferometry (BLl) kinetic assay of 83600-6-PBS against Human PD-L1/CD274 was performed. The affinity constant is 2.46 nM.