Anti-Mouse PD-1/CD279 (J43.1)

PD-1/CD279 Monoclonal Antibody for FC
Cat No. 65120-1-Ig

产品说明书

CloneNo. J43.1

宿主/亚型

Armenian Hamster / IgG

种属反应性

Mouse

应用

FC

CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive FC detected inAnti-CD3/CD28 stimulated mouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65120-1-Ig targets PD-1/CD279 in FC applications and shows reactivity with Mouse samples.

经测试应用 FC Application Description
经测试反应性 Mouse
免疫原 B12 cells, mouse PD-1 cDNA-transfected BHK cells 种属同源性预测
宿主/亚型 Armenian Hamster / IgG
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 1
别名 CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1
GenBank蛋白编号BC119179
基因名称 PD-1
Gene ID (NCBI) 18566
RRIDAB_3670274
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDQ02242
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for PD-1/CD279 antibody 65120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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