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  • KD/KO Validated

PCYT2 Polyclonal antibody

PCYT2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14827-1-AP

Print datasheet

Synonyms

ET, PCYT2



经过测试的应用

Positive WB detected inhuman liver tissue, HepG2 cells, MCF-7 cells, mouse testis tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14827-1-AP targets PCYT2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PCYT2 fusion protein Ag6616 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphate cytidylyltransferase 2, ethanolamine
Synonyms ET, PCYT2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000351
Gene Symbol PCYT2
Gene ID (NCBI) 5833
RRIDAB_2159298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99447
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ethanolamine-phosphate cytidylyltransferase (PCYT2) catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PMID:9083101, PMID:31637422). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PMID:9083101).

实验方案

Product Specific Protocols
WB protocol for PCYT2 antibody 14827-1-APDownload protocol
IF protocol for PCYT2 antibody 14827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine.

Authors - Guotong Fu
  • KO Validated
humanIHC

Int J Mol Sci

Cancer Malignancy Is Correlated with Upregulation of PCYT2-Mediated Glycerol Phosphate Modification of α-Dystroglycan.

Authors - Fumiko Umezawa
human,mouseIF,WB

Int J Biol Macromol

p75NTR enhances cognitive dysfunction in a mouse Alzheimer's disease model by inhibiting microRNA-210-3p-mediated PCYT2 through activation of NF-κB

Authors - Zhongliang Wei
  • KD Validated
humanWB

Evid Based Complement Alternat Med

Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis.

Authors - Shanshan Nie