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验证数据展示

  • IF Staining of HepG2 using 83004-4-RR (same clone as 83004-4-PBS)

    IF Staining of HepG2 using 83004-4-RR (same clone as 83004-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83004-4-RR (same clone as 83004-4-PBS)

    FC experiment of HeLa using 83004-4-RR (same clone as 83004-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCSK9 Recombinant antibody (83004-4-RR, Clone:230536E4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83004-4-PBS

    FC experiment of HeLa using 83004-4-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Mouse PCSK9 Recombinant monoclonal antibody, PBS Only (83004-4-PBS, Clone:230536E4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IF Staining of mouse liver using 83004-4-RR (same clone as 83004-4-PBS)

    IF Staining of mouse liver using 83004-4-RR (same clone as 83004-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 83004-4-RR (same clone as 83004-4-PBS)

    IF Staining of mouse liver using 83004-4-RR (same clone as 83004-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83004-4-RR

    Affinity and Kinetic Characterization of 83004-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83004-4-RR against Mouse PCSK9 were performed. The affinity constant is 384 nM.

Mouse PCSK9 Recombinant monoclonal antibody, PBS Only

PCSK9 Uni-rAb® Recombinant Antibody for IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 83004-4-PBS

产品说明书

CloneNo. 230536E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

230536E4, AI415265, AI747682, FH3, HCHOLA3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83004-4-PBS targets PCSK9 in IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg0640

Product name: Recombinant Mouse PCSK9 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 35-694 aa of NM_153565.2

Sequence: QDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVVLMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVEVYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMSQSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPEDQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPRANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRSRRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWTLTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRSRPSAKASWVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proprotein convertase subtilisin/kexin type 9
别名 230536E4, AI415265, AI747682, FH3, HCHOLA3
计算分子量75KD
GenBank蛋白编号NM_153565.2
基因名称 Pcsk9
Gene ID (NCBI) 100102
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ80W65
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCSK9 (Proprotein convertase subtilisin/kexin type 9) is the ninth member of the proprotein convertase family that regulates lipoprotein homeostasis and altered PCSK9 expression was reportedly associated with tumor development and progression. PCSK9 is primarily synthesized and expressed in the liver but also in many other tissues, such as the intestinal tract, kidney, brain, heart, and blood vessels (PMID: 36242053).

实验方案

Product Specific Protocols
FC protocol for PCSK9 antibody 83004-4-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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